Abcam, ab97952, Recombinant Human RND3 protein

Size: 100µg
Recombinant Human RND3 protein is a Human Full Length protein, in the 1 to 241 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, Mass Spec.
Key facts
Purity:>90% SDS-PAGE,
Expression system:Escherichia coli,
Tags:His tag N-Terminus,
Applications:Mass Spec, SDS-PAGESee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:No,
Accession:P61587,
Animal free:No,
Carrier free:No,
Species:Human,
Storage buffer:pH: 8Constituents: 40% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.316% Tris HCl, 0.0292% EDTA, 0.0154% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
RND3 also known as RhoE is a small GTPase with a molecular mass of approximately 25 kDa. It belongs to the Rho family of GTPases. RND3 lacks intrinsic GTPase activity which makes it constantly bound to GTP. This protein gets expressed in several tissues including the brain heart and skeletal muscles. By interfering with the actin cytoskeleton RND3 regulates cell shape and movement.
Biological function summary
The functions of RND3 involve the regulation of the cytoskeleton contributing to processes such as cell adhesion and migration. RND3 operates independently not forming part of a larger protein complex yet it plays a pivotal role in modulating the activity of other signaling molecules. Its influence on the cytoskeleton makes it an important player in maintaining cellular architecture and dynamics which is fundamental for tissue integrity and repair.
Pathways
RND3 is intricately involved in the MAPK and PI3K/AKT signaling pathways. These pathways are essential for cell growth migration and survival. RND3 interacts with proteins such as ROCK1 and ROCK2 in these pathways affecting downstream activities positively or negatively. The regulation of these signaling cascades by RND3 shows its importance in cellular responses to environmental cues.
RND3 is linked to cancer and cardiovascular diseases. Alterations in RND3 expression or function can contribute to the uncontrolled cell proliferation seen in various cancers. In cancer its interactions with signaling proteins like AKT lead to enhanced survival signaling. In the context of cardiovascular diseases RND3 influences vascular smooth muscle cell behavior impacting atherosclerosis progression. Through these connections RND3 emerges as a significant target for therapeutic research.