Abcam, ab98085, Recombinant Human RHOC protein

Size: 50µg / 250µg
Recombinant Human RHOC protein is a Human Full Length protein, in the 1 to 190 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, Mass Spec.
Key facts
Purity:>90% SDS-PAGE,
Expression system:Escherichia coli,
Tags:His tag N-Terminus,
Applications:SDS-PAGE, Mass SpecSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:No,
Accession:P08134,
Animal free:No,
Carrier free:No,
Species:Human,
Storage buffer:pH: 8Constituents: 20% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.316% Tris HCl, 0.0154% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
RHOC also known as ras homolog family member C is a small GTPase with a molecular mass of approximately 22 kDa. This protein part of the Rho family is involved in various cellular functions including motility and mitosis. RHOC is widely expressed in different tissues but shows higher levels in certain tumor cells. Through its role in actin cytoskeleton organization RHOC mediates cellular structure and activity by binding to GDP and GTP molecules thereby controlling cell morphology and movement.
Biological function summary
RHOC influences cell migration invasion and proliferation key processes in cancer progression. It functions within the Rho family of GTPases interacting with other proteins like ROCK and mDia to regulate cytoskeletal dynamics. RHOC does not operate alone; it works as part of signaling complexes that affect cellular mechanisms such as adhesion and vesicle trafficking. Its activity modulates the balance between cell stability and movement making it significant in tissue remodeling and repair.
Pathways
RHOC plays pivotal roles in the Rho/ROCK and PI3K/Akt signaling pathways. Through these pathways it interacts with related GTPases like RHOA and RAC1. In the Rho/ROCK pathway RHOC influences actin cytoskeleton rearrangement impacting cell contractility and motility. Via the PI3K/Akt pathway RHOC is linked to cell survival and growth involving other proteins like PTEN and mTOR highlighting its function in integrating external signals for cellular response.
RHOC is relevant in cancer and cardiovascular diseases. In cancer elevated RHOC expression correlates with increased invasiveness and metastasis particularly in breast and pancreatic cancers. This association involves proteins such as integrins and cadherins which aid in cell adhesion and migration. RHOC is also implicated in cardiovascular disorders where abnormal cell migration and proliferation contribute to atherosclerosis. Understanding these interactions assists in potential targeting of RHOC for therapeutic purposes.