Abcam, ab99292, Anti-PRP6/ANT-1 antibody

Size: 100µL
Rabbit Polyclonal PRP6/ANT-1 antibody. Suitable for IHC-P, IP, WB and reacts with Human, Mouse samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human PRPF6 aa 1-50.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Human,
Applications:WB, IHC-P, IPSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human PRPF6 aa 1-50. The exact immunogen used to generate this antibody is proprietary information.O94906

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 6.8 - 7.4Preservative: 0.09% Sodium azideConstituents: Tris buffered saline, 0.1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
PRP6 also known as ANT-1 is a protein involved in pre-mRNA splicing. Its molecular mass is approximately 111 kDa. PRP6 is expressed ubiquitously across different tissues but shows higher expression in cells with high transcriptional activity. It functions as a part of the spliceosome a large complex responsible for the removal of introns from pre-mRNA. The protein's structure consists of distinct domains that mediate interactions with other spliceosomal components which ensures proper assembly and function.
Biological function summary
This protein has a significant role in regulating gene expression at the post-transcriptional level. PRP6 forms part of the U4/U6-U5 tri-snRNP complex in the spliceosome. This complex plays an important role during spliceosome assembly and the activation process. By facilitating accurate splicing PRP6 influences the diversity of proteins produced by cells. It interacts directly with other proteins like ANT-Anti which contribute to its function.
Pathways
Several key processes involve PRP6's function. It integrates into the splicing pathway which is essential for mRNA maturation. PRP6 is related to proteins like ANT1s which regulates energy metabolism and homeostasis. In addition PRP6 participates in pathways that control the cellular response to different growth factors and environmental signals. This role links PRP6 to the regulation of other important cellular functions including cell growth and differentiation.
Research indicates that impairment in PRP6 function may associate with certain types of cancer and splicing-related genetic disorders. Altered splicing patterns often lead to oncogenic transformations connecting PRP6 to cancer development through pathways involving proteins such as ANT-Anti. Moreover mutations or malfunctions in splicing factors like PRP6 can result in various neurodegenerative diseases highlighting its critical role in maintaining transcriptome integrity.