Abcam, ab99362, Anti-RTF1 antibody

Size: 100µL
Rabbit Polyclonal RTF1 antibody. Suitable for IHC-P, IP, WB and reacts with Mouse, Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human RTF1.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Human,
Applications:IHC-P, WB, IPSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human RTF1. The exact immunogen used to generate this antibody is proprietary information.Q92541

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 6.8 - 7.4Preservative: 0.09% Sodium azideConstituents: Tris buffered saline, 0.1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
RTF1 also known as RNA polymerase II-associated factor 1 homolog is a subunit of the PAF1 complex involved in transcription regulation. The protein has a molecular mass of approximately 105 kDa and is expressed in various tissues including the brain heart and lung. Mechanically RTF1 interacts with the RNA polymerase II complex and facilitates transcription elongation by modifying chromatin structure and participating in histone modification. This action makes RTF1 integral in the regulation of gene expression.
Biological function summary
RNA polymerase II-associated factor 1 plays an important role by promoting transcriptional elongation mRNA processing and histone methylation. It is a critical part of the PAF1 complex which also includes CDC73 CTR9 LEO1 and PAF1 proteins. This association aids the modulation of gene expression by influencing the transition from transcription initiation to elongation and additionally affects RNA processing mechanisms through interaction with other protein factors facilitating the correct synthesis and maturation of mRNA.
Pathways
Several cellular processes involve RNA polymerase II-associated factor 1 including the Wnt signaling pathway and the histone modification pathway. The protein's participation in Wnt signaling impacts cellular proliferation and differentiation. In the context of histone modifications RTF1 is linked to the deposition of histone methylation marks working closely with related proteins like PAF1 and CDC73. These pathways highlight the interconnectedness of RTF1 within cellular machinery.
RTF1 alterations have associations with cancer and neurodegenerative diseases. Dysregulation of the PAF1 complex notably through changes in RTF1 activity has been observed in several cancers where abnormal transcriptional control is a feature. Neurodegenerative disorders also show a link with defects in RTF1 possibly due to impaired transcriptional regulation. In these conditions the interaction and co-regulation with proteins like CDC73 contribute to a deeper understanding of the pathogenesis involved.