Abcam, ab99998, Human Decorin ELISA Kit (DCN)

Size: 1 x 96Tests
Human Decorin ELISA Kit (DCN) is a sandwich ELISA designed to quantify Human Decorin (DCN) with a sensitivity of 1.5 pg/mL. - Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader - Wide dynamic range - quantifies 0.96 - 700 pg/mL
Key facts
Detection method:Colorimetric,
Sample types:Plasma, Cell culture supernatant, Serum,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:< 1.5 pg/mL,
Range:0.96 - 700 pg/mL,
Assay Platform:Microplate

Product details:
Human Decorin ELISA Kit (DCN) ab99998 is a sandwich ELISA to measure Human Decorin (DCN) in serum, plasma, cell culture supernatant with a sensitivity of 1.5 pg/ml.
How the assay works
This assay employs an antibody specific for Human Decorin coated on a 96-well plate. Standards and samples are pipetted into the wells and Decorin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti Human Decorin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Decorin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Assay Specificity
Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details
Human Decorin ELISA Kit (DCN) ab99998 protocol summary
1. Add standard or sample to each well used. Incubate at room temperature
2. Wash and add prepared biotinylated antibody to each well. Incubate at room temperature.
3. Wash and add prepared streptavidin solution. Incubate at room temperature.
4. Add TMB Development Solution to each well. Incubate at room temperature.
5. Add Stop Solution to each well. Read at 450nm immediately.
Optimisation may be required with urine samples.
The standard is affinity purified.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C