Agilent, 5064-8264, ZORBAX 300SB-C18 5µm, 0.5 x 150 mm

ZORBAX 300SB-C18 5µm, 0.5 x 150 mm. A reversed-phase C18 HPLC column with fully porous ZORBAX particles. StableBond technology ensures stability at low pH.

Specifications:
Brand: ZORBAX 300SB-C18
Hardware: SS
Inner Diameter (ID): 0.5 mm
LC Platform: Stainless Steel
Length: 150 mm
Maximum Temperature: 90 °C
Part Number Application Domain: Biopharma
Part Number Application: Monoclonal Antibodies (mABs)PeptidesProteins
Particle Size: 5 µm
Particle Type: Fully Porous
Phase: 300SB-C18
Pore Size: 300 Å
Pressure Rating: 400 bar
Separation Mode: Reversed Phase
Shipping Solvent: Acetonitrile/Water
Technique: LCLC & LC/MS
UNSPSC Code: 41115709
pH Range: 1-8

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Designed for demanding reversed-phase high-performance liquid chromatography (HPLC) applications, this column features a stable-bond C18 stationary phase and a particle size of 5 micrometers, engineered for consistent performance and durability. With a narrow internal diameter of 0.5 mm and a length of 150 mm, it delivers enhanced sensitivity for small sample volumes, making it ideal for the analysis of peptides, small proteins, and other biomolecules.

Its high pore size of 300 angstroms supports the separation of large molecules, expanding its suitability for proteomics, pharmaceutical research, and peptide mapping studies. The advanced silica technology ensures minimal phase bleed, superior peak shapes, and reproducible results across batches.

Compatible with a wide range of Agilent HPLC and UHPLC systems, including both analytical and micro-flow configurations, this column integrates effectively within existing workflows. It is also well-suited for coupling with mass spectrometry for applications requiring high resolution and sensitivity.

The robust end-capping technology minimizes silanol activity, reducing nonspecific interactions and ensuring the stability required for reproducible long-term analysis. Researchers can pair this column with ZORBAX 300SB guard columns and other ZORBAX 300SB phases to accommodate complex separation needs. This column is an essential tool for laboratories focused on biomolecular characterization and quantitation.