Agilent, 5190-2509, Agilent Bio SEC-3, 150Å, 4.6 x 150 mm, 3 µm

Agilent Bio SEC-3, 150Å, 4.6 x 150 mm, 3 µm. A size exclusion column for aggregate analysis of peptides and small proteins. Resolving range 0.5-150 kDa.

Specifications:
Brand: Agilent Bio SEC-3
Hardware: SS
Inner Diameter (ID): 4.6 mm
LC Platform: Stainless Steel
Length: 150 mm
Maximum Temperature: 80 °C
Molecular Weight Lower Limit: 500 Da
Molecular Weight Upper Limit: 150000 Da
Part Number Application Domain: Biopharma
Part Number Application: Aggregate & Fragment AnalysisPeptidesProteins
Particle Size: 3 µm
Particle Type: Fully Porous
Phase: SEC
Pore Size: 150 Å
Pressure Rating: 240 bar
Separation Mode: Size Exclusion (SEC)
Shipping Solvent: Phosphate Buffer
Technique: LC
UNSPSC Code: 41115711
pH Range: 2-8.5

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The Agilent Bio SEC-3 150 Angstrom column is designed for high-resolution size exclusion chromatography of biological macromolecules, including proteins, peptides, and biotherapeutics. With a 4.6 x 150 mm format and 3 micron particle size, this column provides precise molecular size separation, making it ideal for the analysis of aggregates, fragments, and oligomeric states in protein characterization workflows. Its uniform pore structure and advanced silica material ensure robust performance, excellent reproducibility, and narrow peak widths even at high sample loads.

This column is particularly suited for quality control, stability studies, and formulation development in biopharmaceutical research. It is compatible with a wide range of aqueous and organic mobile phases, supporting versatile method development across different biomolecule classes. The design optimizes efficiency when used with Agilent HPLC and UHPLC systems, such as the 1260 Infinity II Bio-Inert LC system, while maintaining compatibility with most major liquid chromatography platforms.

Recommended for researchers seeking reliable characterization of monoclonal antibodies, vaccines, and related biologics, this column integrates seamlessly with Agilent’s full line of bioanalytical workflow solutions, including detectors and sample preparation accessories. Its durable construction ensures extended usability, supporting consistent, high-throughput laboratory operations.