Agilent, 5190-2542, Agilent Bio SEC-5, 2000Å, 7.8 x 150 mm, 5 µm

Agilent Bio SEC-5, 2000Å, 7.8 x 150 mm, 5 µm. A size exclusion column for aggregate analysis of very large biological molecules. Resolving range 150 kDa to >10 MDa.

Specifications:
Brand: Agilent Bio SEC-5
Hardware: SS
Inner Diameter (ID): 7.8 mm
LC Platform: Stainless Steel
Length: 150 mm
Maximum Temperature: 80 °C
Molecular Weight Upper Limit: > 10,000,000 Da
Part Number Application Domain: Biopharma
Part Number Application: Aggregate & Fragment AnalysisMonoclonal Antibodies (mABs)PeptidesProteins
Particle Size: 5 µm
Particle Type: Fully Porous
Phase: SEC
Pore Size: 2000 Å
Pressure Rating: 240 bar
Separation Mode: Size Exclusion (SEC)
Shipping Solvent: Phosphate Buffer
Technique: LC
UNSPSC Code: 41115711
pH Range: 2-8.5

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The Agilent Bio SEC-5 column offers high-performance size exclusion chromatography for biomolecule analysis, featuring a 2000 Angstrom pore size and 5 micron particle size within a 7.8 x 150 millimeter dimension. Optimized for the separation and characterization of proteins, monoclonal antibodies, and other large biomolecules, this column delivers accurate molecular size distribution and aggregate analysis with excellent resolution and reproducibility. It is especially suited for use in biopharmaceutical development, quality control, and academic research focusing on protein purification, purity assessment, and formulation studies. The robust silica-based stationary phase offers chemical stability, low non-specific adsorption, and compatibility with a wide range of aqueous mobile phases. This column integrates seamlessly with Agilent InfinityLab and 1100, 1200, or 1260 Infinity II LC systems, as well as other major HPLC platforms. For comprehensive biomolecule analysis, it can be paired with additional Agilent Bio SEC-5 column dimensions or utilized alongside Agilent protein standards and detection modules. Its design supports routine high-throughput workflows, helping researchers ensure the integrity and consistency of biologic therapeutics and protein-based products in both industrial and academic laboratory environments.