Agilent, 5190-2544, Agilent Bio SEC-5, 2000Å, 4.6 x 150 mm, 5 µm

Agilent Bio SEC-5, 2000Å, 4.6 x 150 mm, 5 µm. A size exclusion column for aggregate analysis of very large biological molecules. Resolving range 150 kDa to >10 MDa.

Specifications:
Brand: Agilent Bio SEC-5
Hardware: SS
Inner Diameter (ID): 4.6 mm
LC Platform: Stainless Steel
Length: 150 mm
Maximum Temperature: 80 °C
Molecular Weight Upper Limit: > 10,000,000 Da
Part Number Application Domain: Biopharma
Part Number Application: Aggregate & Fragment AnalysisMonoclonal Antibodies (mABs)PeptidesProteins
Particle Size: 5 µm
Particle Type: Fully Porous
Phase: SEC
Pore Size: 2000 Å
Pressure Rating: 240 bar
Separation Mode: Size Exclusion (SEC)
Shipping Solvent: Phosphate Buffer
Technique: LC
UNSPSC Code: 41115711
pH Range: 2-8.5

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The Agilent Bio SEC-5 column, featuring a 2000 Å pore size, 4.6 x 150 mm dimensions, and 5 µm particle size, is engineered for the efficient size exclusion chromatography of biological macromolecules, including proteins, antibodies, and other biotherapeutics. Its robust silica-based stationary phase and optimized surface chemistry provide exceptional resolution and reproducibility across a wide molecular weight range, making it ideal for molecular weight determination, aggregate analysis, and purity assessment. This column is suited for researchers working in biopharmaceutical development, quality control, or academic laboratories aiming to separate and characterize high molecular weight analytes under native conditions. It is compatible with most HPLC and UHPLC systems, offering seamless integration for existing laboratory workflows. The Agilent Bio SEC-5 can be paired with a variety of detectors such as UV, fluorescence, multi-angle light scattering, or refractive index for advanced analytical capabilities. To further enhance performance, it can be used alongside Agilent’s high-purity mobile phases and sample filtration solutions. Its durability ensures consistent results even with demanding sample matrices, providing a reliable solution for routine biopolymer analysis or cutting-edge research in protein therapeutics and structural biology.