Agilent, 5190-6871, Agilent Bio SEC-5, 300Å, 21.2 x 50 mm, 5 µm

Agilent Bio SEC-5, 300Å, 21.2 x 50 mm, 5 µm. A preparative scale size exclusion column for the purification of biological samples. Resolving range 5-1,250 kDa.

Specifications:
Brand: Agilent Bio SEC-5
Hardware: SS
Inner Diameter (ID): 21.2 mm
LC Platform: Preparative HPLC
Length: 50 mm
Maximum Temperature: 80 °C
Molecular Weight Lower Limit: 5000 Da
Molecular Weight Upper Limit: 1250000 Da
Part Number Application Domain: Biopharma
Part Number Application: Aggregate & Fragment AnalysisMonoclonal Antibodies (mABs)PeptidesProteins
Particle Size: 5 µm
Particle Type: Fully Porous
Phase: SEC
Pore Size: 300 Å
Pressure Rating: 240 bar
Separation Mode: Size Exclusion (SEC)
Shipping Solvent: Phosphate Buffer
Technique: LC
UNSPSC Code: 41115709
pH Range: 2-8.5

**********The following product description is generated by AI and is for reference only. If you have any questions, please contact customer service.**********
The Agilent Bio SEC-5 300A column, featuring dimensions of 21.2 x 50 mm and a 5 micron particle size, is designed for high-resolution size exclusion chromatography (SEC) of biomolecules. This column is optimized for the analysis and purification of proteins, peptides, and other macromolecules under aqueous conditions. Its 300 angstrom pore size enables efficient separation of analytes with molecular weights ranging from small proteins to larger aggregates, making it ideal for applications such as aggregate analysis, molecular weight determination, and formulation studies in biopharmaceutical development.

The silica-based stationary phase provides excellent mechanical stability, high recovery, and reproducible retention times, supporting both preparative and analytical workflows. Compatible with a broad array of liquid chromatography systems, including Agilent InfinityLab and 1100/1200 series instruments, the Bio SEC-5 column integrates seamlessly into established laboratory processes. It is also well-suited for use with a wide range of buffer systems commonly employed in SEC.

Researchers in biotechnology, pharmaceutical development, and protein research rely on this column for its durability, reliable performance, and consistent separation profiles. For expanded method development or scale-up, matching guard columns and larger format columns are also available in the Agilent Bio SEC-5 portfolio, ensuring flexibility across diverse analytical demands.