Agilent, 763973-902, ZORBAX Extend 300 C18, 4.6 x 150 mm, 3.5 µm

ZORBAX Extend 300 C18, 4.6 x 150 mm, 3.5 µm. A reversed-phase C18 HPLC column with fully porous ZORBAX particles. The 300-Extend C18 chemistry incorporates a unique bidentate silane which allows the column to withstand pH up to 11.5.

Specifications:
Brand: ZORBAX
Hardware: SS
Inner Diameter (ID): 4.6 mm
LC Platform: Stainless Steel
Length: 150 mm
Maximum Temperature: 40 °C at pH 8-11.560 °C at pH 6-8
Part Number Application Domain: Biopharma
Part Number Application: Monoclonal Antibodies (mABs)PeptidesProteins
Particle Size: 3.5 µm
Particle Type: Fully Porous
Phase: Extend-C18
Pore Size: 300 Å
Pressure Rating: 400 bar
Separation Mode: Reversed Phase
Shipping Solvent: Acetonitrile/Water
Technique: LCLC & LC/MS
UNSPSC Code: 41115709
pH Range: 2-11

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The ZORBAX Extend 300 C18 column, measuring 4.6 x 150 mm with 3.5 micron particle size, is engineered for high-performance reversed-phase liquid chromatography. It features an extended pH range, enabling robust separation of peptides, proteins, and other biomolecules under both low and high pH conditions. The wider 300 Angstrom pore size facilitates efficient analysis of large molecular weight compounds, making it ideal for proteomics, biopharmaceutical research, and quality control environments. This column delivers reliable batch-to-batch reproducibility, excellent peak shapes, and superior resolution for complex mixtures. Its durable packing is optimized for use with both traditional HPLC and UHPLC systems, supporting a range of Agilent and compatible industry-standard instruments. The extended pH stability enlarges method development options and enhances column longevity. Common applications include peptide mapping, protein characterization, small molecule separations, and other bioanalytical studies. The ZORBAX Extend columns seamlessly integrate with Agilent LC platforms such as the 1100, 1200, and 1290 Infinity Series, as well as comparable systems from other manufacturers. For workflows requiring complementary selectivity or additional dimensions of separation, it is often paired with other ZORBAX phase chemistries. This column supports high-throughput labs seeking reliable performance for demanding biological analyses.