Agilent, 822600-909, Poroshell StableBond 300 C3, 1.0 x 30 mm, 5 µm

Poroshell StableBond 300 C3, 1.0 x 30 mm, 5 µm. A reversed-phase C3 HPLC column with superficially porous particles. StableBond technology ensures stability at low pH.

Specifications:
Brand: Poroshell 300
Hardware: SS
Inner Diameter (ID): 1.0 mm
LC Platform: Stainless Steel
Length: 30 mm
Maximum Temperature: 90 °C
Part Number Application Domain: Biopharma
Part Number Application: Intact & Subunit AnalysisMonoclonal Antibodies (mABs)Proteins
Particle Size: 5 µm
Particle Type: Superficially Porous
Phase: 300SB-C3
Pore Size: 300 Å
Pressure Rating: 400 bar
Separation Mode: Reversed Phase
Shipping Solvent: Methanol/Water
Technique: LC
UNSPSC Code: 41115709
pH Range: 1-8

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This analytical column features a 1.0 x 30 mm dimension with 5 micrometer particle size, utilizing Agilent's advanced StableBond 300 C3 bonded phase on Poroshell silica. It is designed for robust and efficient reversed-phase liquid chromatography, particularly suited for the analysis of hydrophobic peptides, small proteins, and biotherapeutics. The proprietary C3 chemistry ensures high stability at low pH, making it ideal for reproducible separations under challenging mobile phase conditions.

Common applications include peptide mapping, protein digestion analysis, biomolecule quantitation, and impurity profiling in pharmaceutical and biotech research. Its short length and narrow internal diameter enable high-throughput workflows and rapid analyses without compromising resolution.

This column is fully compatible with Agilent LC and LC-MS systems, including 1100, 1200 Infinity, 1260, and 1290 Infinity series, as well as other major manufacturers’ HPLC and UHPLC instruments that support standard 1.0 mm internal diameter columns. It pairs effectively with other StableBond and Poroshell columns for comprehensive method development and transfer.

Precision engineering ensures minimal carryover and consistent performance, making it an excellent choice for demanding laboratories engaged in proteomics, drug discovery, and quality control. Batch-to-batch reproducibility and robust hardware contribute to reliable, long-term operation in regulated environments.