Agilent, 884973-701, ZORBAX GF-250, 4.6 x 250 mm, 4 µm

ZORBAX GF-250, 4.6 x 250 mm, 4 µm. These legacy USP L35 zirconium-stabilized silica-based SEC columns have a pore size of 150 Å to separate proteins ranging from 4 to 400 kDa molecular weight.

Specifications:
Brand: ZORBAX
Hardware: SS
Inner Diameter (ID): 4.6 mm
LC Platform: Stainless Steel
Length: 250 mm
Maximum Temperature: 60 °C
Molecular Weight Lower Limit: 4000 Da
Molecular Weight Upper Limit: 400000 Da
Part Number Application Domain: Biopharma
Part Number Application: Aggregate & Fragment AnalysisPeptidesProteins
Particle Size: 4 µm
Particle Type: Fully Porous
Phase: GF-250
Pore Size: 150 Å
Separation Mode: Size Exclusion (SEC)
Shipping Solvent: Phosphate Buffer
Technique: LC
UNSPSC Code: 41115711
pH Range: 3-8.5

**********The following product description is generated by AI and is for reference only. If you have any questions, please contact customer service.**********
The ZORBAX GF-250 is a size exclusion chromatography column engineered for high-resolution separation of proteins and polymers within a molecular weight range of approximately 5,000 to 250,000 Da. Featuring a 4.6 x 250 mm format with 4 micron particle size, this column offers excellent efficiency and reproducibility, making it ideal for precise molecular size determination. It is widely used in biopharmaceutical quality control, protein purification, aggregation studies, and characterization of synthetic polymers. Its robust silica-based packing ensures stable performance and long column life across repeated runs.

This chromatography column is fully compatible with a wide range of HPLC systems, including Agilent 1100 and 1200 Series instruments, as well as other standard analytical HPLC platforms equipped with compatible column hardware. It works well with aqueous mobile phases commonly used in protein and polymer analysis. For method development or comparison studies, it complements Agilent's broader portfolio of ZORBAX GF columns, such as the GF-450 and GF-250, allowing for scalable and customizable separation strategies. This product is an excellent choice for analytical laboratories seeking high accuracy and reproducibility in molecular weight determination and quality control of biomolecules and polymers.