Agilent, GPPNG-PC, GlykoPrep-plus N-glycan Prep with InstantPC, 96-ct

GlykoPrep-plus N-glycan Prep with InstantPC, 96-ct. Complete kit configured for automation on the Agilent AssayMAP Bravo liquid handling platform. InstantPC dye is an instant glycan labeling reagent for MS that provides increased MS and fluorescent sensitivity, which forms a stable urea linkage with glycosylamines released by rapid N-glycanase (PNGase F) digestion. Cartridge-based workflow and utilizes a rapid digestion, instant labeling, and cleanup of excess InstantPC label. Includes GSP-RX digestion module, GSP-PC InstantPC labeling module, GS96-CU cleanup module, AM96-NG GlykoPrep-plus labware set

Specifications:
Count: 96
Equipment & Reagents Provided by User: 100% Acetonitrile (~250 mL, HPLC-grade)Calibrated pipettes and disposable tips (P5/10, P200 and P1000)Formic acid (HPLC-grade)Glass bottle with screw cap (50 mL, 1 ea)Glass graduated cylinder (50 mL, 1 ea)Nitrile glovesUltrapure, deionized water (~100 mL, Milli-Q or equivalent)Volumetric pipettes (5 mL, 1 ea)Vortexer
Instrument Compatibility: AssayMAP Bravo Platform
Kit Configuration: Sample Prep Kit
Label: InstantPC
Prep time (approx): 3 hours
Technique: LC & LC/MS

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This 96-count kit is designed for efficient release and labeling of N-linked glycans from glycoproteins, streamlining sample preparation in glycomics research. The workflow features an advanced glycan labeling technology that utilizes InstantPC, providing highly sensitive, fluorescent, and MS-active tagged glycans in under two hours. The optimized protocol ensures robust, reproducible glycan profiles suitable for high-throughput applications, including biopharmaceutical characterization, biosimilar comparability, biomarker discovery, and quality control testing.

The kit is compatible with a variety of analytical platforms such as UHPLC, HILIC, CE, and mass spectrometry systems from Agilent and other leading vendors, enabling seamless integration into existing lab workflows. It contains all the necessary reagents and consumables for streamlined, plate-based processing, minimizing sample loss and hands-on time while maximizing yield and reliability. Researchers can confidently analyze glycan structures and abundance in complex biological samples, ensuring detailed characterization and meeting regulatory demands. For extended analytical capabilities, the kit is fully compatible with Agilent's Glycan Mapping HPLC columns, InstantPC detection chemistries, and related sample preparation solutions. This comprehensive approach supports both routine and advanced glycan analysis across academic, clinical, and industrial environments.