Agilent, GX24-401AB, AdvanceBio Gly-X deglycosylation and 2-AB Express labeling module set, 24-ct

AdvanceBio Gly-X deglycosylation and 2-AB Express labeling module set, 24-ct. Utilizes a novel in-solution enzymatic protein deglycosylation followed by Express labeling of released N-glycans with 2-AB dye. With deglycosylation carried out in solution, the method is simple, rapid and suitable for automation. Includes GX24-100 Gly-X deglycosylation module (24-ct), GX24-401 Gly-X 2-AB Express labeling module (24-ct).

Specifications:
Application: Flexible, high-throughput format:process 1 to 96 samples
Count: 24
Deglycosylation Efficiency: > 99 %
Equipment & Reagents Provided by User: 96-well Thermocycler or two independent heat blocksAcetonitrile (ACN), MS-grade (Fisher A955-4 recommended)Gly-X 2-AB Express cleanup module (96-ct) [GX96-402]THERM-1000, 230V)Thermocycler (Corning THERM-1001, 110V
Kit Configuration: Sample Prep Module
Label: 2-AB
Prep time (approx): 130 min (24 samples)
Protein Load Range: 1-40 µg
Recovery: > 80 %
Stability: At least 1 month at -20°C, 5 days at 4–8°C
Technique: LC & LC/MS

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The AdvanceBio Gly-X deglycosylation and 2-AB Express labeling module set is designed to streamline glycan analysis for biopharmaceutical research and quality control. This set enables simultaneous rapid enzymatic deglycosylation and fluorescent 2-AB labeling of N-glycans from glycoproteins, supporting high-throughput sample processing with consistent and reliable results. Optimized for ease of use, the workflow completes in under two hours without the need for complicated manual steps, reducing hands-on time and minimizing errors.

Ideal for applications in therapeutic antibody characterization, vaccine development, and biosimilar comparability studies, this module set provides precise identification and quantitation of glycan profiles. Samples processed with this set are compatible with Agilent AdvanceBio Glycan Mapping columns for both HILIC-UPLC and LC-MS analysis, as well as Agilent fragment analyzers and LC systems, ensuring adaptability within diverse laboratory infrastructures.

Each kit contains enough reagents to process 24 samples, making it suitable for small batch studies or routine testing. The robust protocol ensures accurate and reproducible data, facilitating regulatory submissions and research insights. Use this module set to accelerate glycan workflow, increase throughput, and gain deep structural understanding of glycoproteins in your biopharmaceutical projects.