BD, 340365, BD FastImmune™ Anti-Human CD4 FITC/CD69 PE/CD3 PerCP

Reactivity: Human
Application: Flow cytometry
Regulatory Status: RUO (GMP)
RRID: AB_400415
Storage Buffer: Phosphate buffered saline with BSA and 0.1% sodium azide.
Description: Description CD4, clone SK3, is derived from hybridization of mouse NS-1 myeloma cells with spleen cells from BALB/c mice immunized with human peripheral blood T lymphocytes. CD69, clone L78, is derived from hybridization of mouse Sp2/0-Ag14 myeloma cells with lymph node cells from BALB/c mice immunized with a CD8 + alloantigen-directed cytotoxic T-lymphocyte (CTL) cell line. CD3, clone SK7, is derived from hybridization of mouse NS-1 myeloma cells with spleen cells from BALB/c mice immunized with human thymocytes. CD4 recognizes an antigen that interacts with class II molecules of the major histocompatibility complex (MHC). CD4 is the primary receptor for the human immunodeficiency virus (HIV). The cytoplasmic portion of the antigen is associated with the protein tyrosine kinase p56 lck . The CD4 antigen may regulate the function of theCD3 antigen/T-cell antigen receptor (TCR) complex. CD4 also reacts with monocytes/macrophages that have an antigen density lower than that on helper/inducer T lymphocytes.  CD69 recognizes a very early human lymphocyte activation antigen. The CD69 antigen is a surface homodimer formed by the association of 28-kilodalton (kd) and 32-kd chains that are held together by disulfide bridges. CD3 recognizes the epsilon chain of the CD3 antigen/T-cell antigen receptor (TCR) complex. This complex is composed of at least six proteins that range in molecular weight from 20 to 30 kd. The antigen recognized by the CD3 antibody is noncovalently associated with either α/β or γ/δ TCR (70 to 90 kd).
Preparation And Storage: Preparation And Storage The FastImmune reagent is supplied as a combination of CD4 FITC, CD69 PE, and CD3 PerCP in 1.0 mL of phosphate-buffered saline (PBS) containing bovine serum albumin (BSA) and 0.1% sodium azide. The vial should be stored at 2° to 8°C. Conjugated forms should not be frozen and should be protected from prolonged exposure to light. Each reagent is stable for the period shown on the bottle label when stored as directed.
Description : Clone : Isotype : EntrezGene ID
N/A : N/A : | IgG1, κ,IgG1, κ,IgG1, κ : N/A