BD, 550078, BD Pharmingen™ PE Mouse Anti-Human MIP-1β

Alternative Name: Macrophage inflammatory protein 1-beta; CCL4; C-C motif chemokine 4; LAG-1
Reactivity: Human (QC Testing)
Isotype: Mouse IgG1, κ
Immunogen: Recombinant Human MIP-1β
Application: Intracellular staining (flow cytometry) (Routinely Tested)
Concentration: 0.2 mg/ml
RRID: AB_393549
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Recommended Assay Procedures: Recommended Assay Procedures IF/Flow : For immunofluorescent staining and flow cytometric analysis, the D21-1351 antibody has been found useful to identify and enumerate MIP-1β producing cells within mixed cell populations. PE Mouse Anti-Human MIP-1β (Cat. No. 550078/561120) is especially suitable for these studies. A suitable mouse IgG1 isotype control for assessing the level of background staining on paraformaldehyde-fixed/saponinpermeabilized human cells is PE Mouse IgG1, κ Isotype Control (Cat. No. 554680); use at comparable concentrations to antibody of interest (e.g., . 0.5 µg mAb/1 million cells). A useful control for demonstrating specificity of staining is to pre-block the fixed/permeabilized cells with unlabeled D21-1351 antibody (custom order), prior to staining. The intracellular staining technique and use of blocking controls are described in detail by C. Prussin and D. Metcalfe.
Product Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. An isotype control should be used at the same concentration as the antibody of interest. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.