BD, 550290, BD Pharmingen™ Purified Rat Anti-Mouse CD62E

Alternative Name: Sele; E-selectin; Elam; ELAM-1; LECAM2; LYAM2
Reactivity: Mouse (QC Testing)
Isotype: Rat LEW, also known as Lewis IgG2a, κ
Immunogen: Mouse brain capillary endothelioma bEnd.3 (TNFα-stimulated)
Application: Flow cytometry (Routinely Tested), Immunohistochemistry-frozen (Tested During Development), Immunohistochemistry-formalin (antigen retrieval required) (Not Recommended)
Concentration: 125 µg/ml
RRID: AB_393585
Storage Buffer: Aqueous buffered solution containing BSA, goat serum, and ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Recommended Assay Procedures: Recommended Assay Procedures Immunohistochemistry: The 10E9.6 antibody (Cat. No. 550290) is recommended to test for immunohistochemical staining of acetone-fixed frozen sections. Tissue tested was tongue from LPS treated C57BL/6. The antibody stains endothelial cells in the mouse. The isotype control recommended for use with this antibody is purified rat IgG2a (Cat. No. 559073). For optimal indirect immunohistochemical staining, the 10E9.6 antibody should be titrated (1:10 to 1:50 dilution) and visualized via a three-step staining procedure in combination with biotinylated polyclonal anti-rat Igs (multiple adsorbed) (Cat. No. 559286) as the secondary antibody and Streptavidin-HRP (Cat. No. 550946) together with the DAB detection system (Cat. No. 550880). The clone 10E9.6 is not recommended for formalin-fixed paraffin embedded sections. A detailed protocol of the immunohistochemical procedure is available at our website, http://www.bdbiosciences.com/support/resources