BD, 550298, BD Pharmingen™ Purified Mouse Anti-Rat CD8a

Alternative Name: Cd8a; CD8α; CD8 alpha; OX-8 membrane antigen
Reactivity: Rat (QC Testing)
Isotype: Mouse BALB/c IgG1, κ
Immunogen: High-molecular-weight rat thymocyte glycoproteins
Application: Flow cytometry (Routinely Tested), Immunohistochemistry-formalin (antigen retrieval required), Immunohistochemistry-frozen, Immunohistochemistry-paraffin, Immunohistochemistry-zinc-fixed (Tested During Development), Blocking, Immunoaffinity Chromatography, Immunoprecipitation, Western blot (Reported)
Concentration: 15.625 µg/ml
RRID: AB_2075364
Storage Buffer: Aqueous buffered solution containing BSA, goat serum, and ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Recommended Assay Procedures: Recommended Assay Procedures Immunohistochemistry: The OX-8 antibody is recommended to test for immunohistochemical staining of acetone-fixed frozen sections and paraffin sections. For paraffin sections no pretreatment is required. Tissues tested were rat spleen and thymus. The antibody stains the CD8 subset of T lymphocytes. The isotype control recommended for use with this antibody is purified mouse IgG1 (Cat. No. 550878). For optimal indirect immunohistochemical staining, the OX-8 antibody should be titrated (1:10 to 1:50 dilution) and visualized via a three-step staining procedure in combination with biotinylated polyclonal anti-mouse Ig (multiple adsorbed) (Cat. No. 550337) as the secondary antibody and Streptavidin-HRP (Cat. No. 550946) together with the DAB detection system (Cat. No. 550880). More conveniently, the anti-mouse Ig HRP detection kit (Cat. No. 551011) that contains the biotinylated secondary antibody, antibody diluent, streptavidin-HRP and DAB substrate can be used for staining.