BD, 550381, BD Pharmingen™ Z-LEHD-FMK, Caspase-9 Inhibitor

Application: Flow cytometry (Routinely Tested)
RRID: AB_2868883
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage Avoid multiple freeze-thaws of product. Z-LEHD-FMK, Caspase-9 Inhibitor is provided as a lyophilized powder. Store the lyophilized inhibitor at -20˚C. Reconstitute the inhibitor in DMSO before use. The reconstituted inhibitor may be stored in small aliquots at -20˚C.
Recommended Assay Procedures: Recommended Assay Procedures The Z-LEHD-FMK inhibitor can be used for both in vivo and in vitro cell based assays to measure the inhibition of apoptosis. While there are numerous methods to induce and measure apoptosis, Annexin V staining can be used on Jurkat cells (Human T-cell leukemia; ATCC TIB-152) for evaluating apoptosis (see figure). Reconstitute 1.0 mg of Z-LEHD-FMK in DMSO. A 10 mM stock solution may be prepared by dissolving 1.0 mg of Z-LEHD-FMK in 124 µl DMSO. When using the Z-LEHD-FMK inhibitor, the optimal concentration needed may vary with the experimental system being used and investigators are highly encouraged to titrate the reagent. As a precautionary note, please do not exceed a final DMSO concentration of 0.2% as higher levels may increase the risk for cellular toxicity which may mask the effect of the caspase inhibitor.