BD, 550392, BD Pharmingen™ Purified Mouse Anti-Human CD44

Alternative Name: Phagocytic glycoprotein 1; Pgp-1; H-CAM; Hermes; ECMR III; HUTCH-1
Reactivity: Human (QC Testing)
Isotype: Mouse IgG2b, κ
Application: Flow cytometry (Routinely Tested), Immunohistochemistry-formalin (antigen retrieval required), Immunohistochemistry-frozen (Tested During Development)
Concentration: 250 µg/ml
Workshop Number: VI A092
RRID: AB_2074674
Storage Buffer: Aqueous buffered solution containing BSA, goat serum, and ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Recommended Assay Procedures: Recommended Assay Procedures Immunohistochemistry: The G44-26 antibody is recommended to test for immunohistochemical staining of acetone-fixed frozen sections and formalin-fixed paraffin sections with citrate pre-treatment. Tissues tested were human spleen, thymus and tonsil. The antibody stains thymocytes, leukocytes, erythrocytes and weakly on platelets. The isotype control recommended for use with this antibody is purified mouse IgG2b (Cat. No. 557351). For optimal indirect immunohistochemical staining, the G44-26 antibody should be titrated (1:10 to 1:50 dilution) and visualized via a three-step staining procedure in combination with polyclonal, biotin conjugated anti-mouse Igs (multiple adsorbed) (Cat. No. 550337) as the secondary antibody and Streptavidin-HRP (Cat. No. 550946) together with the DAB detection system (Cat. No. 550880). A detailed protocol of the immunohistochemical procedure is available on our website at http://www.bdbiosciences.com/us/s/resources.