BD, 550428, BD Pharmingen™ Purified Mouse Anti-Human IRF-3

Alternative Name: Interferon regulatory factor 3; IRF-3; IRF3; IIAE7
Reactivity: Human (QC Testing)
Isotype: Mouse IgG1, κ
Immunogen: Human IRF-3 aa. 56-427 Recombinant Fusion Protein
Application: Western blot (Routinely Tested), Blocking, Immunofluorescence, Immunoprecipitation (Reported)
Target Molecular Weight: 50 kDa
Concentration: 0.5 mg/ml
RRID: AB_393677
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Recommended Assay Procedures: Recommended Assay Procedures Clone SL-12.1 can be used for western blot analysis (0.5-2.0 µg/ml). Other reported applications include immunoprecipitation and immunofluorescence microscopy. The antibody will recognize both the nuclear and cytoplasmic forms of IRF-3 and can block IRF-3 DNA binding, although this application has not been tested at BD Biosciences. Jurkat cells are recommended as a positive control (Cat. No. 611451).
Product Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.