BD, 550549, BD Pharmingen™ Purified Rat Anti-Mouse Flk-1

Alternative Name: CD309; Fetal liver kinase 1; Kdr; VEGF Receptor-2; VEGFR-2; AVAS12;
Reactivity: Mouse (QC Testing)
Isotype: Rat WI, also known as Wistar (outbred) IgG2a, κ
Immunogen: Mouse Flk-1 Recombinant Protein
Application: Flow cytometry (Routinely Tested), Immunohistochemistry-frozen (Tested During Development), Immunohistochemistry-formalin (antigen retrieval required) (Not Recommended)
Concentration: 31.25 µg/ml
RRID: AB_2132506
Storage Buffer: Aqueous buffered solution containing BSA, goat serum, and ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Recommended Assay Procedures: Recommended Assay Procedures Immunohistochemistry: The Avas 12α1 antibody specific for mouse Flk-1 is recommended to test for immunohistochemical staining of acetone-fixed frozen sections. Tissues tested were mouse spleen, thymus and gastrointestinal tract. The antibody stains endothelial cells. The isotype control recommended for use with this antibody is Purified Rat IgG2a κ Isotype Control (Cat. No. 559073). For optimal indirect immunohistochemical staining, the Avas 12α1 antibody should be titrated (1:10 to 1:50 dilution) and visualized via a three-step staining procedure in combination with Biotin Goat Anti-Rat Ig (Cat. No. 559286) as the secondary antibody and Streptavidin-HRP (Cat. No. 550946) together with the DAB detection system (Cat. No. 550880). Alternatively, Anti-rat Ig HRP Detection Kit (Cat. No. 551013) offers all of the necessary reagents to stain for this antibody.