BD, 551022, BD Pharmingen™ Purified Mouse Anti- Bcl-x

Application: Western blot (Routinely Tested)
Regulatory Status: RUO
RRID: AB_394007
Description: Description Members of the Bcl-2 family play a major role in regulating the response of cells to a wide variety of apoptotic signals. Some Bcl-2 family members like Bcl-2, block apoptosis, whereas others promote apoptosis and inhibit Bcl-2 activity. Bcl-x is a Bcl-2 homologue that has two isoforms, resulting from alternative splicing. Bcl-xL (long) is a 241 amino acid protein (25-29 kDa) that is 47% homologous to Bcl-2 on the amino acid level. Bcl-xS (short) is a 178 amino acid protein (~ 19.5 kDa) lacking a 63 amino acid domain that is well conserved among members of the Bcl-2 protein family. Bcl-xL blocks cell death, whereas Bcl-xS inhibits Bcl-2 and promotes cell death. The 2H12 antibody has been reported to recognize human, mouse and rat Bcl-xL (long) protein. An N-terminal peptide (amino acids 3-14) common to human and mouse Bcl-xL and Bcl-xS (short) proteins was used as the immunogen. Thus the antibody is also predicted to recognize the Bcl-xS protein as the sequence used for the immunogen is common to both Bcl-xL and Bcl-xS proteins. Reports from development, however, have indicated that Bcl-xS has not been observable.
Preparation And Storage: Preparation And Storage Store undiluted at -20°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Recommended Assay Procedures: Recommended Assay Procedures In addition to Jurkat cell lysates,  MOLT-4 (Human T-lymphoblasts; ATCC CRL-1582), K-562 (Human bone marrow myelogenous leukemia; ATCC CCL-243), Hut-78 (Human T lymphoma; ATCC TIB-161), human peripheral blood mononuclear cells (PBMC), rat thymocytes and mouse thymocytes have also been reported to be useful as positive controls.