BD, 552503, BD Pharmingen™ Biotin Mouse Anti-Human CD126

Alternative Name: IL-6 Receptor α Chain
Reactivity: Human (QC Testing)
Isotype: Mouse BALB/c IgG1, κ
Immunogen: Mixture of U266, XG-1and BWD41 cells
Application: ELISA Detection (Routinely Tested)
Concentration: 0.5 mg/ml
Workshop Number: V C019, VI C-87
RRID: AB_2127927
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed. Store undiluted at 4°C.
Recommended Assay Procedures: Recommended Assay Procedures ELISA Detection: The Biotinylated M182 antibody (Cat. No. 552503) is useful as a detection antibody for a sandwich ELISA measuring human IL-6Ra protein levels. Biotinylated M182 antibody can be paired with the purified M5 anti-human IL-6Ra (Cat. No. 551462) as the capture antibody, with recombinant soluble IL-6Ra (please inquire) as the standard. Biotin M182 antibody should be titrated between 0.5-2 µg/ml to determine its optimal concentration for ELISA capture. To obtain linear standard curves, doubling dilutions of recombinant human soluble IL-6Ra, ranging from 500 to 2 pg/ml are recommended for inclusion in each ELISA plate. For specific methodology, see BD Pharmingen's Immune Function Handbook, which is posted on our website at www. BDBiosciences.com. Note: This ELISA antibody pair shows no cross-reactivity with the following recombinant human cytokines: CD40, CD40L, CD14, IL-1Ra, IL-1R1, IL-1RII, IL-1α, IL-1ß, IL-2, IL-3, IL-4, sIL-4R, IL-5, IL-6, IL-7, IL-8, IL-10, IL-12p40, IL-12p70, IL-13, IL-15, TNF-α, TNF-ß, sTNFRI, sTNFRII, IFNγ, TRAIL, GM-CSF, TGFß, Trx.