BD, 552857, BD Pharmingen™ Purified Rat Anti-Mouse VEGFR-3

Reactivity: Mouse (QC Testing)
Isotype: Rat IgG2a, κ
Immunogen: VEGFR-3 extracellular domain
Application: Immunohistochemistry-paraffin (Routinely Tested), Immunohistochemistry-frozen (Tested During Development)
RRID: AB_394498
Storage Buffer: Aqueous buffered solution containing BSA, goat serum, and ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Recommended Assay Procedures: Recommended Assay Procedures Immunohistochemistry: The AFL4 antibody, specific for mouse VEGFR-3, is tested for immunohistochemical staining of acetone-fixed frozen sections and zinc-fixed and formalin-fixed, paraffin-embedded sections. Antigen retrieval with BD Retrievagen A (Cat. No. 550524) is recommended for formalin-fixed, paraffin sections. Tissues tested were mouse spleen, liver, kidney, lung and small intestine. The antibody stains endothelial cells of lymphatic vessels. The isotype control recommended for use with this antibody is purified rat IgG2aκ (Cat. No. 559073). For optimal indirect immunohistochemical staining, the AFL4 antibody should be titrated (1:10 to 1:50 dilution) and visualized via a three-step staining procedure in combination with polyclonal, biotin conjugated anti-rat Igs (multiple adsorbed) (Cat. No. 559286) as the secondary antibody and Streptavidin-HRP (Cat. No. 550946) together with the DAB Detection System (Cat. No. 550880). More conveniently, the Anti-Rat Ig HRP Detection Kit (Cat. No. 551013), which contains the biotinylated secondary antibody, antibody diluent, streptavidin-HRP and DAB substrate, can be used for staining.