BD, 552958, BD Pharmingen™ Purified Rat Anti-Mouse F4/80-Like Receptor

Alternative Name: Fire; Emr4; EGF-like module receptor 4; D17Ertd479e; Egf-tm7
Reactivity: Mouse (QC Testing)
Isotype: Rat IgG2a, κ
Immunogen: CHO cells expressing recombinant FIRE fusion protein
Application: Flow cytometry (Routinely Tested), Immunohistochemistry-frozen (Reported), Immunoprecipitation, Western blot (Not Recommended)
Concentration: 0.5 mg/ml
RRID: AB_394533
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.
Recommended Assay Procedures: Recommended Assay Procedures For flow cytometry of leukocyte suspensions, we recommend that Mouse BD Fc Block™ purified anti-mouse CD16/CD32 mAb 2.4G2 (Cat. No. 553141/553142) be used. Since this antigen is expressed at low density on myeloid cells, it may be desirable to amplify staining by using a biotinylated second-step antibody followed by a "bright" third-step reagent, such as Streptavidin-APC (Cat. No. 554067). If Mouse BD Fc Block™ antibody is used, it is important that the second-step anti-rat IgG does not react with 2.4G2 mAb (rat IgG2b, κ); we recommend biotinylated anti-rat IgG2a mAb RG7/1.30 (Cat. No. 553894).