BD, 553000, BD Pharmingen™ Purified Rat Anti-Mouse CD90.2

Alternative Name: Thy-1.2; T25; Thymus cell antigen 1, theta
Reactivity: Mouse (QC Testing)
Isotype: Rat LOU, also known as Louvain, LOU/C, LOU/M IgG2a, κ
Immunogen: Mouse Thymus / Spleen
Application: Flow cytometry (Routinely Tested), Immunohistochemistry-frozen (Tested During Development), Cytotoxicity, Electron microscopy, Immunoprecipitation (Reported)
Concentration: 0.5 mg/ml
RRID: AB_394539
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.
Recommended Assay Procedures: Recommended Assay Procedures Flow Cytometry: Mouse BD Fc Block™ purified anti-mouse CD16/CD32 clone 2.4G2 (Cat. No. 553141) may help to reduce non-specific binding to cells bearing Fcγ-receptors.  For staining in the presence of Mouse BD Fc Block™, a second step antibody which does not recognize the 2.4G2 mAb (Rat IgG2b, κ) must be used, such as FITC anti-rat IgG2a, mAb RG7/1.30 (Cat. No. 553896). Caution: Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also effect the results of functional studies, we recommend the NA/LE™ (No Azide/Low Endotoxin) antibody format for in vitro and in vivo use.
Product Notices: Product Notices Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols. Since applications vary, each investigator should titrate the reagent to obtain optimal results.