BD, 553027, BD Pharmingen™ Purified Rat Anti- Mouse CD8a

Alternative Name: Cd8a; CD8 alpha chain; Ly-2; Lyt2; Lyt-2; Ly-35; Ly-B
Reactivity: Mouse (QC Testing)
Isotype: Rat LOU, also known as Louvain, LOU/C, LOU/M IgG2a, κ
Immunogen: Mouse Spleen Cells or Thymocyte Membranes
Application: Flow cytometry (Routinely Tested), Immunohistochemistry-frozen, Immunohistochemistry-zinc-fixed (Tested During Development), Blocking, Depletion, Functional assay, Immunoprecipitation (Reported), Immunohistochemistry-formalin (antigen retrieval required) (Not Recommended)
Concentration: 0.5 mg/ml
RRID: AB_394565
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE™ antibody format (Cat.No. 553026) for in vitro and in vivo use.
Recommended Assay Procedures: Recommended Assay Procedures This antibody is routinely tested by flow cytometric analysis.  Since applications vary, each investigator must determine dilutions appropriate for individual use.  For IHC, we recommend use of purified 53-6.7 mAb in our special formulation for immunohistochemistry (Cat. No. 550281). IHC of formalin-fixed paraffin-embedded sections is not recommended. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.
Product Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. An isotype control should be used at the same concentration as the antibody of interest. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.