BD, 553088, BD Pharmingen™ FITC Rat Anti-Mouse CD45R/B220

Alternative Name: B220; Ly-5; CD45R; LCA; Ptprc; Protein tyrosine phosphatase receptor type C
Reactivity: Mouse (QC Testing), Human (Reported)
Isotype: Rat IgG2a, κ
Immunogen: Mouse Abelson Leukemia Virus-Induced pre-B tumor cells
Application: Flow cytometry (Routinely Tested), Immunofluorescence (Reported)
Concentration: 0.5 mg/ml
RRID: AB_394618
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The antibody was conjugated to the dye under optimum conditions and unconjugated antibody and free dye were removed.
Recommended Assay Procedures: Recommended Assay Procedures BD® CompBeads can be used as surrogates to assess fluorescence spillover (Compensation).  When fluorochrome conjugated antibodies are bound to CompBeads, they have spectral properties very similar to cells.   However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls.  It is strongly recommended that when using a reagent for the first time, users compare the spillover on cell and CompBead to ensure that BD® CompBeads are appropriate for your specific cellular application.