BD, 553387, BD Pharmingen™ Purified Rat Anti-Mouse IgG2a

Reactivity: Mouse (QC Testing)
Isotype: Rat LOU, also known as Louvain, LOU/C, LOU/M IgG1, κ
Immunogen: Pooled BALB/c and C57BL/6 mouse Ig
Application: ELISA (Routinely Tested)
Concentration: 0.5 mg/ml
RRID: AB_394825
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.
Recommended Assay Procedures: Recommended Assay Procedures For the sandwich mouse IgG2a ELISA, biotin-, AKP-, or HRP-conjugated R19-15 mAb (Cat. no. 553388, 553389, or 553391, respectively) is optimal for detection with purified anti-mouse IgG2a R11-89 mAb (Cat. no. 553446) for capture. This pair of anti-mouse IgG2a mAbs can effectively quantitate mouse IgG2a of these mouse Igh-C allotypes, in order of sensitivity from highest to lowest: e, a, j, and b . R19-15 antibody is effective for detection of cell-surface Ig by immunofluorescent staining with flow cytometric analysis. For flow cytometric detection of intracytoplasmic IgG2a, we recommend FITC-conjugated mAb R19-15 (Cat. no. 553390).