BD, 553406, BD Pharmingen™ Biotin Rat Anti-Mouse IgM

Alternative Name: Ighm; Igh-M; Immunoglobulin M; Igh6; muH; immunoglobulin heavy constant mu
Reactivity: Mouse (QC Testing)
Isotype: Rat LOU, also known as Louvain, LOU/C, LOU/M IgG2a, κ
Immunogen: Pooled Mouse Ig
Application: ELISA (Routinely Tested), Flow cytometry (Tested During Development)
Concentration: 0.5 mg/ml
RRID: AB_394843
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.
Recommended Assay Procedures: Recommended Assay Procedures For the sandwich mouse IgM ELISA, biotin-conjugated R6-60.2 mAb is optimal for detection with purified anti-mouse IgM II/41 mAb (Cat. No. 553435) for capture. Biotinylated R6-60.2 mAb (Cat. No. 553406) may be used as a primary or secondary reagent in immunofluorescent staining. Biotinylated R6-60.2 mAb may be used as a primary or secondary reagent in immunofluorescent staining. For flow cytometric detection of intracytoplasmic IgM, we recommend FITC-conjugated mAb II/41 (Cat. No. 553437).