BD, 553455, BD Pharmingen™ Biotin Mouse IgG2a, κ Isotype Control

Isotype: Mouse BALB/c IgG2a, κ
Immunogen: TNP-keyhole limpet hemocyanin
Application: Flow cytometry, Isotype control (Routinely Tested), Intracellular staining (flow cytometry) (Not Recommended)
Concentration: 0.5 mg/ml
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Recommended Assay Procedures: Recommended Assay Procedures An isotype control should be used at the same concentration as the antibody of interest (e.g., ≤ 1 µg/million cells for flow cytometry). We recommend Cat. No. 555572 for immunofluorescent staining of human whole blood.
Product Notices: Product Notices Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols. Since applications vary, each investigator should titrate the reagent to obtain optimal results.