BD, 553719, BD Pharmingen™ Purified Hamster Anti-Mouse CD152

Alternative Name: Ctla4; CTLA-4; Cytotoxic T-lymphocyte-associated protein 4; Ly-56
Reactivity: Mouse (QC Testing)
Isotype: Armenian Hamster IgG1, κ
Immunogen: Mouse CTLA-4 IgG2a Fusion
Application: Flow cytometry (Routinely Tested), Immunoprecipitation, Intracellular staining (flow cytometry) (Tested During Development)
Concentration: 0.5 mg/ml
Entrez Gene ID: 12477
RRID: AB_395004
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.
Recommended Assay Procedures: Recommended Assay Procedures Since CD152 is expressed at low density on activated T cells, it may be necessary to amplify the signal by using a biotinylated second-step reagent, followed by a "bright" third-step reagent. We have found that biotin-conjugated mouse anti-hamster IgG (Cat. No. 554010) plus Streptavidin-PE (Cat. No. 554061) are effective. Mouse BD Fc Block™ (anti-mouse CD16/CD32 mAb 2.4G2, Cat. No. 553141/553142) may help to reduce non-specific binding of the antibody to cells bearing Fcγ receptors. Since a large proportion of the CTLA-4 molecule is intracellular, detection of the antigen is enhanced by staining cells permeabilized with the BD Cytofix/Cytoperm™ intracellular staining kit (Cat. No. 554714).