BD, 553837, BD Pharmingen™ Purified Armenian Hamster Anti-Mouse CD29

Alternative Name: Itgb1; Integrin beta-1; Fnrb; gpIIa; VLA-4 subunit beta
Reactivity: Mouse (QC Testing), Rat (Tested in Development)
Isotype: Armenian Hamster IgG2, λ1
Immunogen: Purified mouse VLA-4
Application: Flow cytometry (Routinely Tested), Blocking, Immunoprecipitation, Inhibition (Reported)
Concentration: 0.5 mg/ml
RRID: AB_395077
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.
Recommended Assay Procedures: Recommended Assay Procedures Other reported applications include immunoprecipitation and in vitro inhibition (in combination with a mAb to mouse CD11a) of T-cell proliferative responses to anti-CD3e mAb 145-2C11 (Cat. no. 553057) and allogeneic cells. For in vitro blocking of adhesion of mouse or rat CD29-expressing cells to extracellular matrix proteins, we recommend the No Azide/Low Endotoxin (NA/LE™) format of mAb Ha2/5 (Cat. no. 555002). We recommend our immunohistochemistry formulation of purified HMß1-1 mAb, Cat. no. 550530, for immunohistochemical staining (IHC) of rat tissues. For IHC of mouse tissues, we recommend the use of purified anti-mouse CD29 mAb 9EG7 in our special formulation for immunohistochemistry, Cat. no. 550531.
Product Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols. Although hamster immunoglobulin isotypes have not been well defined, BD Biosciences Pharmingen has grouped Armenian and Syrian hamster IgG monoclonal antibodies according to their reactivity with a panel of mouse anti-hamster IgG mAbs. A table of the hamster IgG groups, Reactivity of Mouse Anti-Hamster Ig mAbs, may be viewed at http://www.bdbiosciences.com/documents/hamster_chart_11x17.pdf. Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.