BD, 553956, BD Pharmingen™ APC Hamster IgG1, λ1 Isotype Control

Alternative Name: Anti-TNP
Isotype: Armenian Hamster IgG1, λ1
Immunogen: Trinitrophenol-KLH
Application: Flow cytometry, Isotype control (Routinely Tested)
Concentration: 0.2 mg/ml
RRID: AB_398540
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to APC under optimum conditions, and unconjugated antibody and free APC were removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Recommended Assay Procedures: Recommended Assay Procedures This immunoglobulin is useful as an isotype-matched negative control for immunofluorescent staining. An isotype control should be used at the same concentration as the antibody of interest (e.g., ≤ 1 µg/million cells for flow cytometry). The APC fluorochrome is excited by laser lines from 595 to 647 nm, and its emission is collected in a detector for fluorescence wavelengths between 640 and 680 nm.