BD, 554267, BD Pharmingen™ Purified Mouse Anti-Human FLI-1

Reactivity: Human (QC Testing)
Isotype: Mouse IgG2a
Immunogen: Fli-1 ets Domain Fusion Protein
Application: Western blot (Routinely Tested), Gel shift, Immunoprecipitation (Tested During Development)
Target Molecular Weight: 50 kDa
Concentration: 0.5 mg/ml
RRID: AB_395334
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.
Recommended Assay Procedures: Recommended Assay Procedures Applications include western blot analysis (0.5-2 µg/ml). Additional applications not routinely tested at BD Biosciences Pharmingen include gel shift and immunoprecipitation. Clone G146-254 has been shown to recognize in vitro translated, recombinant bacterially expressed, and endogenous B cell Fli-1. In gel shift assays this antibody supershifts complexes of Fli-1 and DNA probe.
Product Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.