BD, 554268, BD Pharmingen™ Purified Mouse Anti-Human PU.1

Reactivity: Human (QC Testing)
Isotype: Mouse IgG2a
Immunogen: PU.1 Fusion Protein
Application: Western blot (Routinely Tested), Gel shift, Immunoprecipitation (Tested During Development)
Target Molecular Weight: 40-42 kDa
Concentration: 0.5 mg/ml
RRID: AB_395335
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.
Recommended Assay Procedures: Recommended Assay Procedures Applications include gel shift, western blot analysis (1-2 µg/ml) and immunoprecipitation. The antibody has been shown to recognize in vitro translated PU.1 and PU.1 expressed as a recombinant protein in bacteria. In. In gel shift assays using µB as a probe (µB is a transcriptional element in the immunoglobulin heavy chain enhancer), G148-74 supershifts the complex of probe and PU.1.