BD, 554689, BD Pharmingen™ PE Rat IgG2a, κ Isotype Control

Isotype: Rat LOU, also known as Louvain, LOU/C, LOU/M IgG2a, κ
Immunogen: Mouse Pooled Immunoglobulin
Application: Flow cytometry, Intracellular staining (flow cytometry), Isotype control (Routinely Tested)
Concentration: 0.2 mg/ml
RRID: AB_479724
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Recommended Assay Procedures: Recommended Assay Procedures An isotype control should be used at the same concentration as the antibody of interest (e.g., ≤ 0.5 µg/million cells for flow cytometry). The intracellular cytokine staining technique and use of blocking controls are described in detail by C. Prussin and D. Metcalfe. For specific methodology, please visit our web site, www.bdbiosciences.com, and refer to the protocols section or the chapter on intracellular staining in the Immune Function Handbook.