BD, 555709, BD Pharmingen™ Biotin Mouse Anti-Human CD23

Reactivity: Human (QC Testing)
Isotype: Mouse IgG1, κ
Application: Flow cytometry (Routinely Tested), ELISA Detection (Tested During Development)
Concentration: 0.5 mg/ml
Workshop Number: V CD23.15
RRID: AB_396055
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Recommended Assay Procedures: Recommended Assay Procedures ELISA Detection: This biotinylated M-L233 antibody is useful as a detection antibody in ELISA for measuring soluble human CD23 protein levels in combination with the purified mouse anti-human sCD23, clone M-L234 as the capture antibody. This biotinylated detection antibody should be pretitrated in the range of 0.5 to 5 µg/ml to determine its optimal concentration for ELISA. IF/Flow: This antibody can be used for immunofluorescent staining and flow cytometric analysis in conjunction with streptavidin (or avidin)-fluorochrome second step reagent.