BD, 556917, BD Pharmingen™ Purified Mouse Anti-Human IL-4

Reactivity: Human (QC Testing)
Isotype: Mouse IgG1, κ
Immunogen: Recombinant Human IL-4
Application: ELISA Capture (Routinely Tested), Intracellular block/flow cytometry (Tested During Development)
Concentration: 0.5 mg/ml
RRID: AB_398620
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Recommended Assay Procedures: Recommended Assay Procedures 1. Blocking Control for Intracellular Staining: The purified 8D4-8 antibody (Cat. No. 556917) can be used as a blocking control to demonstrate specificity of IL-4 staining by conjugated-8D4-8 antibodies. The intracellular cytokine staining technique and use of blocking controls are described in detail by C. Prussin and D. Metcalfe. For specific methodology, please visit the protocols section or chapter on intracellular staining in the Immune Function Handbook, both of which are posted on our web site, www.bdbiosciences.com. 2. ELISA Capture: The purified 8D4-8 antibody (Cat. No. 554515) is useful as a capture antibody for a sandwich ELISA for measuring human IL-4 protein levels. Purified 8D4-8 antibody can be paired with the biotinylated MP4-25D2 antibody (Cat. No. 554483) as the detecting antibody, with recombinant human IL-4 (Cat. No. 554605) as the standard. For testing IL-4 in serum or plasma, the OptEIA™  human IL-4 ELISA set (Cat. No. 555194) or OptEIA™ human IL-4 ELISA kit (Cat. No. 550614) are recommended.