BD, 558294, BD Pharmingen™ PE Rat Anti-Mouse CD284/MD-2 Complex

Alternative Name: TLR4/MD-2 Complex
Reactivity: Mouse (QC Testing)
Isotype: Rat IgG2a, κ
Immunogen: Mouse Pro-B cell line Ba/F3 expressing transfected BALB/c mouse TLR4 and MD-2
Application: Flow cytometry (Routinely Tested)
Concentration: 0.2 mg/ml
RRID: AB_397073
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Recommended Assay Procedures: Recommended Assay Procedures Mouse BD Fc Block™ purified anti-mouse CD16/CD32 mAb 2.4G2 (Cat. No. 553141/553142) may help to reduce non-specific binding to cells bearing Fcγ receptors. If Mouse BD Fc Block™ is used, then it is important that the second-step anti-rat IgG antibody does not cross-react with the 2.4G2 mAb (Rat IgG2b, κ); we recommend the use of biotinylated anti-rat IgG2a mAb RG7/1.30 (Cat. No. 553894) with a "bright" third-step reagent, such as Streptavidin-PE (Cat. No. 554061).
Product Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.