BD Horizon™ APC-R700 Mouse Anti-Human HLA-DR 565127

Alternative Name: MHC class II antigen; HLA class II histocompatibility antigen
Reactivity: Human (QC Testing), Rhesus, Cynomolgus, Baboon (Tested in Development), Dog (Reported)
Isotype: Mouse IgG2a, κ
Immunogen: Human lymphoblastoid B-cell line RPMI 8866
Application: Flow cytometry (Routinely Tested)
Vol. Per Test: 5 µl
RRID: AB_2732055
Storage Buffer: Aqueous buffered solution containing BSA, protein stabilizer, glycerol and ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon APC-R700 under optimum conditions, and unconjugated antibody and free BD Horizon APC-R700 were removed.
Product Notices: Product Notices This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test). An isotype control should be used at the same concentration as the antibody of interest. Source of all serum proteins is from USDA inspected abattoirs located in the United States. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors. Species cross-reactivity detected in product development may not have been confirmed on every format and/or application. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.