BD Pharmingen™ APC Mouse Anti-Human CD3 570820

Alternative Name: CD3-epsilon; CD3E; Leu4; T-cell surface antigen T3/Leu-4 epsilon chain; T3E
Reactivity: Human (QC Testing)
Isotype: Mouse BALB/c IgG1, κ
Immunogen: Human Thymocytes
Application: Flow cytometry (Routinely Tested)
Vol. Per Test: 5 µl/test
Workshop Number: II T118; III T492
Entrez Gene ID: 916
RRID: AB_3686060
Storage Buffer: Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions and unconjugated antibody and free dye were removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Recommended Assay Procedures: Recommended Assay Procedures BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation).  When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells.   However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls.  It is strongly recommended that when using a reagent for the first time, users compare the spillover on cell and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.
Product Notices: Product Notices Please refer to www.bdbiosciences.com/us/s/resources for technical protocols. Source of all serum proteins is from USDA inspected abattoirs located in the United States. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test). This APC-conjugated reagent can be used in any flow cytometer equipped with a dye, HeNe, or red diode laser. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors. An isotype control should be used at the same concentration as the antibody of interest. Please refer to http://regdocs.bd.com to access safety data sheets (SDS). For U.S. patents that may apply, see bd.com/patents.