Bio-Rad, 1560123, Profinity™ IMAC Resin, uncharged, 50 ml

Pkg of 1, 50 ml, support with uncharged iminodiacetic acid (IDA) for immobilized metal affinity chromatography
Overview
Use Profinity IMAC Resin for purification of recombinant histidine-tagged proteins. Optimized ligand density and open pore structure provides high-stringency binding of his-tagged and metal-binding proteins for a wide range of molecular weights.
• Chelating ligand is iminodiacetic acid (IDA)
• Can be used in batch protocols or with gravity, low-pressure, and medium-pressure chromatography systems
• Stable across a wide pH range (1–14)
• Works under native or denaturing conditions
• Can be used for single-step purification from crude samples or contaminant removal
Profinity IMAC Resin is compatible with denaturants (including 8 M urea and 6 M guanidine HCl), detergents, reducing agents, alcohols, chelating agents, salts, and other common protein purification reagents.
Product Options
Prepacked Bio-Scale™ Mini Profinity™ IMAC Cartridges with uncharged or Ni-charged IDA, and Bio-Scale™ Mini Nuvia™ IMAC Cartridges with Ni-charged NTA are available.
Related Products
• Native IMAC Buffer Kit ( 6200239 ) includes IMAC lysis, wash, and elution buffers
• Native IMAC Purification Kits, 1 ml ( 6200241 ) includes Native IMAC buffer Kit and 2x1 ml Bio-Scale Mini IMAC Cartridges, and 5 ml ( 6200242 ) includes Native IMAC buffer Kit and 1x5 ml Bio-Scale Mini IMAC Cartridge
• Nuvia IMAC Resin , Ni-charged nitrilotriacetic acid (NTA)
• Empty Bio-Scale Mini Cartridges, 1 ml ( 7324660 ) and 5 ml ( 7324661 )
• Poly-Prep ® Chromatography Columns , hold up to 2 ml support
• His Antibody ( 6200203 )
Related Categories
• NGC™ Medium-Pressure Liquid Chromatography Systems
• BioLogic™ Low-Pressure Liquid Chromatography Systems
• Profinia™ Affinity Chromatography Protein Purification System
• Transfection
• Spectrophotometry
Supporting Documents
• Profinity IMAC Resins Instruction Manual
• Profinity IMAC Resin Product Information Sheet
• Profinity IMAC Ni-Charged Resin Flier
• Profinity IMAC Resin: An Optimal Support for Histidine-Tagged Protein Purification Tech Note
• Bio-Scale Mini Profinity IMAC Cartridges, 1 and 5 ml Instruction Manual
• Bio-Scale Mini Profinity IMAC Cartridge Product Information Sheet
Online Resources
• Introduction to Chromatography
• Introduction to Transfection
• Histidine-Tagged Recombinant Protein Purification and On-Column Refolding