Bio-Rad, 1610771, 10x Tris/Glycine Buffer for Western Blots and Native Gels

Pkg of 1, 5 L cube, 10x premixed electrophoresis buffer contains 25 mM Tris, 192 mM glycine, pH 8.3 following dilution to 1x with water
Overview
Use 10x Tris/Glycine Buffer as a transfer buffer for western blots or as a running buffer for native protein gel electrophoresis.
• For tank or semi-dry blotting for SDS PAGE gels, usually with the addition of 20% methanol
• For tank blotting of native gels, without methanol
• As a running buffer for native gels
Precast gels that can be used for native electrophoresis
Note: Most proteins have an acidic or slightly basic pI (~3–8) and are run with the power supply connected to the electrophoresis chamber as for SDS-PAGE. If more basic proteins (pl >8.5) of interest are being separated, change the polarity of the electrodes, since they have a net positive charge.
Packaging Options
Related Products
• PrecisionAb™ Validated Western Blotting Antibodies
• Native Sample Buffer ( 1610738 )
• Criterion Staining/Blotting Trays with lids ( 3459921 )
• 10x Phosphate Buffered Saline (PBS) ( 1610780 )
• 10x Tris Buffered Saline (TBS) (1706435)
• 1x Phosphate Buffered Saline (PBS) with 1% Casein ( 1610783 )
• 1x Tris Buffered Saline (TBS) with 1% Casein ( 1610782 )
• Blotting-Grade Blocker, nonfat dry milk ( 1706404 )
Related Categories
• Protein Gels
• Western Blotting Membranes and Filter Paper
• Semi-Dry and Rapid Blotting Systems
• Wet/Tank Blotting Systems
• Power Supplies
• Transfer Buffers
• Immunodetection Reagents and Kits
• Gel and Western Imaging Systems
Supporting Documents
• Mini-PROTEAN Precast Gels Instruction Manual and Application Guide
• Criterion Gel Application Guide Instruction Manual
• Mini Trans-Blot ® Cell Instruction Manual
• Trans-Blot SD Cell Quick Reference Guide
• Trans-Blot SD Semi-Dry Transfer Cell Instruction Manual
Online Applications
• Protein Electrophoresis
• Western Blotting
• Imaging and Analysis