Bio-Rad, 1633006, ProteoMiner™ Protein Enrichment Small-Capacity Kit

10 preps, low-abundance protein enrichment kit for 10 mg of total protein, includes 10 spin columns, wash buffer, elution reagents, collection tubes
Overview
Use ProteoMiner Protein Enrichment Small-Capacity Kits to simultaneously concentrate low-abundance proteins and lower the concentration of high-abundance proteins in ≥10 mg of sample. The protein complexity of the sample is maintained.
The enrichment protocol uses a large, highly diverse library of combinatorial peptide ligands bound to beads. After high-abundance proteins have saturated all their high-affinity ligands, the unbound excess protein is washed away. Most medium- and low-abundance proteins will not saturate their ligands, resulting in no excess to be washed away.
• Knowledge of the identity and characteristics of the proteins within a sample is not required
• Compatible with a wide range of sample types
• Narrows the range of protein concentrations within a sample
• Can be used for differential expression analysis
• Compatible with most downstream applications including 1-D and 2-D electrophoresis and mass spectrometry
For fractionation of the protein sample after binding to the beads, the ProteoMiner Protein Enrichment Small-Capacity Kit can be used with the ProteoMiner Sequential Elution Reagents ( 1633003 ). The elution kit contains four different elution buffers, providing multiple options for successive elution of populations of proteins with different characteristics. The ProteoMiner Protein Enrichment Small-Capacity Kit and Sequential Elution Reagents can be purchased together in one kit, the ProteoMiner Sequential Elution Small-Capacity Kit ( 1633010 ). Sequential elution has been optimized to prepare samples for SELDI ProteinChip analysis. Elution reagents are not compatible with direct use of fractions in 2-D gel electrophoresis.
Kit Contents
• Spin columns, 10; 500 µl bead slurry (4% beads, 20% v/v EtOH), 20 µl settled bead volume
• Wash Buffer, 150 mM NaCl, 10 mM NaH 2 PO 4 , pH 7.4, 50 ml
• Elution Reagent, lyophilized 8 M urea, 2% CHAPS, 2 vials
• Rehydration Reagent, 5% acetic acid, 5 ml
• Capless 2 ml centrifuge tubes, 20
• Capped 2 ml centrifuge tubes, 10
• Instruction Manual
• Quick Start Guide
Product Options
Related Products
• Mini Bio-Spin ® Chromatography Columns ( 7326207 )
• ReadyPrep™ Proteomics Grade Water ( 1632091 )
• ReadyPrep 2-D Cleanup Kit
Related Categories
• Centrifuges
• Protein Electrophoresis and Blotting
• 2-D Electrophoresis
• Protein Assay Kits and Cuvettes
• Protein Fractionation
• Magnetic Beads and Racks for Immunoprecipitation
Supporting Documentation
• ProteoMiner Protein Enrichment and Sequential Kit Protocols
• ProteoMiner Sequential Elution Kit Protocol
• ProteoMiner Protein Enrichment Kits Instruction Manual
• ProteoMiner Protein Enrichment Technology Brochure
• Enrichment of Medium- and Low-Abundance Proteins in Sample Types Using ProteoMiner Technology Application Note