Bio-Rad, 1725150, iTaq™ Universal SYBR ® Green One-Step Kit, 100 x 20 µl rxns, 1 ml

100 x 20 µl reactions, 1 ml (1 x 1 ml), one-step RT-PCR kit, includes 2x one-step SYBR ® Green reaction mix containing iTaq DNA polymerase, buffers, and ROX normalization dyes; iScript™ reverse transcriptase; and nuclease-free water
Overview
Our Universal Real-Time PCR Reagents Web App brings you in-depth product information, individual and competitive performance data, interactive tutorials, 3-D animated videos, technical manuals, and more.
Use our combined iScript™ RNase H+reverse transcriptase and antibody hot-start iTaq DNA polymerase kit formulation to complete your SYBR ® Green real-time reactions in one step – PCR efficiency is improved over a wide dynamic range and under various conditions.
• Advanced formulation for amplifying difficult targets and challenging samples
• Can be combined with our PrimePCR™ assays for a simplified workflow
• Universal — works on any real-time PCR system
Kit Components
• Reverse transcriptase – 25 µl
• Reaction mix – 1 ml (1 x 1 ml)
Instrument Compatibility
iTaq Universal SYBR Green One-Step RT-qPCR Kit is compatible with Bio-Rad Real-Time PCR Detection Systems and commercially available systems listed below:
• StepOne/StepOnePlus
• 7500, ViiA 7
• 7000, 7300, 7700, 7900HT, QuantStudio Systems
• Mx3000P, 3005P, 4000
• Mastercycler ep realplex 2, 4
• Corbett Rotor-Gene 3000, 6000, Q
• LightCycler 480, 96
• LightCycler 1.0, 1.5, 2.0*
• LightScanner HR-1
• LightScanner 32*
* BSA must be added according to instrument specifications
Applications and Uses of the iTaq ® Universal SYBR ® Green One-Step Kit
• qPCR/real-time PCR
• Gene expression analysis
• Absolute quantification
• Mutation detection
• Pathogen detection
• Viral detection (load)
• Genetically modified organism (GMO) characterization
• Genetic profiling
Packaging Options
The iTaq™ universal SYBR ® Green one-step kit is also available in a format of 500 reactions (171-5151) .
Related Products
Bio-Rad has also developed one-step kits for probe-specific applications .