Biolegend, 100233, Brilliant Violet 510™ anti-mouse CD3 Antibody, 125microl

CD3, also known as T3, is a member of the Ig superfamily and primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation. CD3 is composed of CD3ε, δ, γ and ζ chains. It forms a TCR complex by associating with TCR α/β or γ/δ chains. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding the peptide/MHC antigen complex
125microl
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: γδTCR-positive T-T hybridoma D1
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 510™ under optimal conditions.
Concentration: µg sizes: 0.2 mg/mLµL sizes: lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining using the µg size, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. For flow cytometric staining using the µl size, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.Brilliant Violet 510™ excites at 405 nm and emits at 510 nm. The bandpass filter 510/50 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 510™ is a trademark of Sirigen Group Ltd.Learn more about Brilliant Violet™. This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser: Violet Laser (405 nm)
Application Notes: Additional reported application (for relevant formats) include: spatial biology (IBEX)1,2.
Application References(PubMed link indicates BioLegend citation): Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations: Cheng C, et al. 2022. Cell Mol Gastroenterol Hepatol. 15:261. PubMed Bhaskar A, et al. 2023. iScience. 26:106644. PubMed Yao RQ, et al. 2023. Mil Med Res. 10:27. PubMed Su Y, et al. 2022. J Hematol Oncol. 15:99. PubMed Orvain C, et al. 2022. Arthritis Res Ther. 24:13. PubMed Liang Y, et al. 2022. Theranostics. 12:7729. PubMed Yang H, et al. 2023. Cell Death Differ. 30:560. PubMed Takacs GP, et al. 2023. Front Immunol. 13:993444. PubMed Zhang B, et al. 2023. Signal Transduct Target Ther. 8:28. PubMed Reticker-Flynn NE, et al. 2022. Cell. 185:1924. PubMed Lechuga-Vieco AV, et al. 2020. Sci Adv. 6:eaba5345. PubMed Platt DJ, et al. 2021. Cell Reports. 35(6):109113. PubMed Wang L, et al. 2019. Cell Rep. 29:1848. PubMed Hirata Y et al. 2018. Cell stem cell. 22(3):445-453 . PubMed Zhang YS, et al. 2018. Cancer Biol Ther. 19:735. PubMed Stegelmeier AA, et al. 2022. Biomedicines. 10:. PubMed Delacher M, et al. 2021. Immunity. 54(4):702-720.e17. PubMed Feizi N, et al. 2021. Cell Death Dis. 12:1026. PubMed Menzel L, et al. 2021. Cell Rep. 37:109878. PubMed Okamoto T, et al. 2020. Cancer Res. 3580:80. PubMed Reyes RM, et al. 2021. Oncoimmunology. 10:2006529. PubMed Xiong A, et al. 2022. EBioMedicine. 83:104239. PubMed Ciecko AE, et al. 2019. Cell Rep. 29:3073. PubMed Qiu F, et al. 2022. J Cancer. 13:2893. PubMed Kuhn NF, et al. 2020. Nat Commun. 4.74375. PubMed Bittner–Eddy PD, et al. 2017. Front Immunol. 1.304166667. PubMed Zhang B, et al. 2021. Nat Biomed Eng. 5:1288. PubMed Wang F, et al. 2018. Carcinogenesis. 39:889. PubMed Hu Y, et al. 2022. J Nanobiotechnology. 20:417. PubMed Mara AB, et al. 2022. NPJ Vaccines. 7:86. PubMed Sordé L, et al. 2017. Immunity, Inflammation, and Disease. 10.1002/iid3.167. PubMed Landon J Edgar et al. 2018. Cell chemical biology. 26(1):131-136 . PubMed Hombrink P, et al. 2016. Nat Immunol. 17:1467-1478. PubMed Wijewarnasuriya D, et al. 2020. Cancer Immunol Res. 0.841666667. PubMed Cabrera-Perez J, et al. 2016. J Immunol. 197: 1692 - 1698. PubMed Cabrera-Perez C, et al. 2015. J Immunol . 194:1609-20. PubMed Fernández-Orth J, et al. 2020. Eur J Immunol. . PubMed Lu YJ, et al. 2021. Cell Rep. 36:109696. PubMed Cerina M, et al. 2017. Brain Behav Immun. 59:103-117. PubMed Heil J, et al. 2021. Nat Commun. 12:6963. PubMed Bahmani B, et al. 2021. Nat Commun. 12:1999. PubMed Hu Y, et al. 2021. J Nanobiotechnology. 19:416. PubMed Li X, et al. 2021. Front Immunol. 12:779560. PubMed Yang F, et al. 2021. Nat Commun. 12:3424. PubMed Ren X, et al. 2021. Cell Death Dis. 12:484. PubMed
RRID: AB_2561387 (BioLegend Cat. No. 100233) AB_2562555 (BioLegend Cat. No. 100234)
Structure: Ig superfamily, CD3/TCR, 20 kD
Distribution: Thymocytes (differentiation dependent), mature T cells, NK-T cells
Function: Antigen recognition, TCR signal transduction, T cell activation
Ligand/Receptor: Peptide antigen/MHC-complex
Antigen References: 1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Davis MM. 1990. Annu. Rev. Biochem. 59:475. 3. Weiss A, et al. 1994. Cell 76:263.
Gene ID: 12502
UniProt: View information about CD3 on UniProt.org
Clone: 17A2
Regulatory Status: RUO
Other Names: T cell antigen receptor complex, T3
Isotype: Rat IgG2b, κ