Biolegend, 100328, PerCP/Cyanine5.5 anti-mouse CD3ε Antibody, 100μg

CD3ε is a 20 kD transmembrane protein, also known as CD3 or T3. It is a member of the Ig superfamily and primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation. CD3ε forms a TCR complex by associating with the CD3δ, γ and ζ chains, as well as the TCR α/β or γ/δ chains. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding the peptide/MHC antigen complex.
100μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Armenian Hamster
Immunogen: H-2Kb-specific mouse cytotoxic T lymphocyte clone BM10-37
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is = 0.25 µg per 106 cells in 100 µl. It is recommended that the reagent be titrated for optimal performance for each application.* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
Excitation Laser: Blue Laser (488 nm)
Application Notes: Clone 145-2C11 is useful for in vitro blocking of target-specific CTL-mediated cell lysis1, as well as T cell activation assays, inducing proliferation and cytokine production1,2,7,12,16. It also induces apoptosis in immature thymocytes32, and in vivo T cell depletion8-10. Additional reported applications (for relevant formats of this clone) include: immunoprecipitation1, immunohistochemical staining14,15 of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections, Western blotting4, complement-mediated cytotoxicity6, in vitro and in vivo stimulation of T cells1,2,7,12,16, immunofluorescent staining5, and in vivo T cell depletion8-10. The 145-2C11 antibody has been reported to block the binding of 17A2 antibody to CD3 epsilon-specific T cells11. Clone 145-2C11 is not recommended for formalin-fixed paraffin embedded sections. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 100314). For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 100340) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).
Application References(PubMed link indicates BioLegend citation): Leo O, et al. 1987. P. Natl. Acad. Sci. USA 84:1374. (IP, Activ, Block) Kruisbeek AM, et al. 1991. In Current Protocols in Immunology. 3.12.1. (Activ) Duke RC, et al. 1995. Current Protocols in Immunology. 3.17.1. Salvadori S, et al. 1994. J. Immunol. 153:5176. (WB) Payer E, et al. 1991. J. Immunol. 146:2536. (IF) Jacobs H, et al. 1994. Eur. J. Immunol. 24:934. (CMCD) Vossen ACTM, et al. 1995. Eur. J. Immunol. 25:1492. (Activ) Henrickson M, et al. 1995. Transplantation 60:828. (Deplete) Kinnaert P, et al. 1996. Transpl. Int. 9:386. (Deplete) Han WR, et al. 1999. Transpl. Immunol. 7:207. (Deplete) Miescher GC, et al. 1989. Immunol. Lett. 23:113. (Block) Terrazas LI, et al. 2005. Intl. J. Parasitology. 35:1349. (Activ) Ko SY, et al. 2005. J. Immunol. 175:3309. Podd BS, et al. 2006. J. Immunol. 176:6532. (IHC-F) Tilley SL, et al. 2007. J. Immunol. 178:3208. (IHC-F) Wang W, et al. 2007. J. Immunol. 178:4885. (Activ) Xiao S, et al. 2007. J. Exp. Med. 204:1691. Chappaz S, et al. 2007. Blood doi:10.1182/blood-2007-02-074245. (FC) PubMed. Curtsinger JM, et al.2005. J. Immunol. 175:4392. PubMed Guo Y, et al. 2008. Blood 112:480. PubMed Kenna TJ, et al. 2008. Blood 111:2091. Perchonock CE, et al. 2007. J. Immunol. 179:1768. PubMed Perchonock GE, et al. 2006. Mol. Cell. Biol. 26:6005. PubMed Kanaya T, et al. 2008. Am. J. Physiol. Gastrointest. Liver Physiol. 295:G273. PubMed de Koning BA, et al. 2006. Int. Immunol. 18:941. PubMed Schulteis RD, et al. 2008. Blood 295:G273. PubMed Qi Q, et al. 2009. Blood 114:564. PubMed Helmersson S, et al. 2013. Am J Pathol. 9440:123. Pubmed Wu S, et al. 2014. Clin Vaccine Immunol. 21:156. PubMed Yan J, et al. 2014. Vaccine. 32:2833. PubMed Guiterrez DA, et al. 2014. Diaebetes. 63:3827. PubMed Shi YF, et al. 1991. J Immunol. 146:3340. (Apop)
Product Citations: Duarte J, et al. 2012. J Immunol. 189:1680. PubMed Guo H, Cooper S, Friedman A, et al. 2017. PLoS One. 10.1371/journal.pone.0150809. PubMed Cheng C, et al. 2022. Cell Mol Gastroenterol Hepatol. 15:261. PubMed Gao X, et al. 2023. Front Immunol. 14:1142428. PubMed Iwanami N, et al. 2023. Nat Commun. 14:3645. PubMed Miki H, et al. 2020. J Immunol. 204:1892. PubMed Fu A, et al. 2022. Cell. 185:1356. PubMed Grégoire C, et al. 2022. Immunity. 55:1216. PubMed Que W, et al. 2022. Sci Adv. 8:eabo4413. PubMed Liang Y, et al. 2022. Theranostics. 12:7729. PubMed Kulkarni R, et al. 2023. Life Sci Alliance. 6: . PubMed Garcia-Fabiani MB, et al. 2020. Methods Enzymol. 632:369. PubMed Marco Barros R, et al. 2016. Cell. 167: 203-218. PubMed Frisbee AL, et al. 2019. Nat Commun. 10:2712. PubMed Hastings AK, et al. 2019. iScience. 13:339. PubMed Wu SY, et al. 2022. Mol Cancer. 21:84. PubMed Feola S, et al. 2022. Front Immunol. 13:826164. PubMed Geng Z, et al. 2021. Nat Commun. 12:6584. PubMed Wu Q, et al. 2021. Cancer Commun (Lond). Online ahead of prin. PubMed Muthumani K, et al. 2015. Sci Transl Med. 7: 301ra132. PubMed Engler J, et al. 2017. Proc Natl Acad Sci U S A. 114(2):E181-E190. PubMed Fu B et al. 2017. Immunity. 47(6):1100-1113 . PubMed Girousse A et al. 2019. Cell Rep. 27(2):323-333 . PubMed Kobayashi T, et al. 2019. Cell. 176:982. PubMed Wu L, et al. 2022. Front Immunol. 13:860761. PubMed Helmersson S, et al. 2013. Am J Pathol. 182:1671. PubMed Di Luccia B, et al. 2020. Cell Host & Microbe. 27(6):899-908. PubMed Wu M, et al. 2021. Nat Commun. 12:3500. PubMed Liao Y, et al. 2017. Virol J. 10.1186/s12985-017-0801-x. PubMed Das S, et al. 2019. Oncogene. 383316. PubMed Pérez‐Mazliah D et al. 2017. EBioMedicine. 24:216-230 . PubMed LaFleur MW, et al. 2019. Nat Immunol. 20:1335. PubMed Yang W, et al. 2020. Mucosal Immunol. 13:788. PubMed Bertocchi A, et al. 2021. Cancer Cell. 39(5):708-724.e11. PubMed Tritama E, et al. 2018. Hum Vaccin Immunother. 14:1524. PubMed Vijay R, et al. 2015. J Exp Med. 212: 1851 - 1868. PubMed Katakam A, et al. 2015. Proc Natl Acad Sci U S A. 112: 14664 - 14669. PubMed Bennion BG, et al. 2020. Cell Reports. 31(11):107771. PubMed Snyder LM, et al. 2022. Immunohorizons. 6:660. PubMed Abu El Maaty MA, et al. 2022. Sci Adv. 8:eabo2295. PubMed Sellau J, et al. 2016. Sci Rep. 6:28058. PubMed Härdle L, et al. 2015. Sci Rep. 5: 15112. PubMed Cong J et al. 2018. Cell metabolism. 28(2):243-255 . PubMed Muñoz NM, et al. 2022. Sci Rep. 12:14449. PubMed McFarlane AJ, et al. 2017. J Allergy Clin Immunol. 140:1068. PubMed Repáraz D, et al. 2022. J Immunother Cancer. 10:. PubMed Glaros V, et al. 2021. Immunity. 54:2005. PubMed McFarland AP, et al. 2021. Immunity. 54(6):1320-1337.e4. PubMed Wu S, et al. 2014. Clin Vaccine Immunol. 21:156. PubMed Chen Y, et al. 2022. Front Immunol. 13:835690. PubMed Uddin MJ, et al. 2022. Mucosal Immunol. 15:165. PubMed Li H, et al. 2018. Nat Commun. 9:2830. PubMed Duan H, et al. 2021. J Clin Invest. 131:. PubMed Sibilio A, et al. 2022. iScience. 25:103790. PubMed Feng J,et al. 2017. Nat Commun. . 10.1038/s41467-017-01056-8. PubMed Wang R, et al. 2022. Front Immunol. 13:947756. PubMed Li Y, et al. 2021. Cell Death Dis. 12:1001. PubMed Muthalagu N, et al. 2020. Cancer Discov. 1.022222222. PubMed Su W et al. 2019. Cancer Cell. 36(2):139-155 . PubMed Lebel MÈ, et al. 2020. Nat Commun. 3.051388889. PubMed Lou Y, et al. 2021. Int J Mol Sci. 22:. PubMed Zhang P, et al. 2022. Nat Commun. 13:1588. PubMed Han Y, et al. 2022. Int J Biol Sci. 18:5653. PubMed Matsumura T, et al. 2022. Nat Commun. 13:7064. PubMed Sakamoto K, et al. 2021. Immunity. 54:2321. PubMed Ko HJ, et al. 2020. Front Immunol. 1897:11. PubMed Mah-Som AY, et al. 2021. Cell Reports. 35(9):109209. PubMed Yan J, et al. 2014. Vaccine. 32:2833. PubMed Zhu K, et al. 2022. EMBO Rep. 23:e54499. PubMed Wang R, et al. 2022. J Immunother Cancer. 10:. PubMed Wagner AK, et al. 2022. iScience. 25:105137. PubMed Bonavita E, et al. 2020. Immunity. 1215:53. PubMed Loo Yau H, et al. 2021. Molecular Cell. 81(7):1469-1483.e8. PubMed Oh B, et al. 2017. Plast Reconstr Surg Glob Open. 5:e1595. PubMed Peng V, et al. 2022. Nat Immunol. 23:619. PubMed Chen B, et al. 2021. Cell. 184:6262. PubMed Teng F, et al. 2021. Cell Rep. 37:110051. PubMed Sakamoto K, et al. 2022. STAR Protoc. 3:101052. PubMed Spiljar M, et al. 2021. Cell Metab. 33:2231. PubMed Zhang M, et al. 2022. iScience. 25:104490. PubMed Zhang B, et al. 2021. Front Neurol. 607370:12. PubMed Young K, et al. 2021. Cell Stem Cell. . PubMed Gutierrez D, et al. 2014. Diabetes. 63:3827. PubMed Thomson CA, et al. 2018. J Immunol. 201:215. PubMed Hodgson R, et al. 2022. Commun Biol. 5:1216. PubMed Zhang L, et al. 2021. Mol Ther. 29:744. PubMed
RRID: AB_893320 (BioLegend Cat. No. 100327) AB_893318 (BioLegend Cat. No. 100328)
Structure: Ig superfamily, forms CD3/TCR complex with CD3δ, γ and ζ subunits and TCR (α/β and γ/δ), 20 kD
Distribution: Thymocytes (differentiation dependent), mature T cells, NK-T cells
Function: TCR signal transduction, T cell activation, antigen recognition
Ligand/Receptor: Peptide antigen/MHC-complex
Cell Type: NKT cells, T cells, Thymocytes, Tregs
Biology Area: Immunology
Molecular Family: CD Molecules, TCRs
Antigen References: 1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Davis MM. 1990. Annu. Rev. Biochem. 59:475. 3. Weiss A, et al. 1994. Cell 76:263.
Gene ID: 12501
UniProt: View information about CD3epsilon on UniProt.org
Clone: 145-2C11
Regulatory Status: RUO
Other Names: CD3ε, T3, CD3
Isotype: Armenian Hamster IgG
Q: How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?
A: PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.